Enhancer and promoter activity in the JH to IGHM intron of the Pekin duck, Anas platyrhynchos.

@article{Lundqvist2007EnhancerAP,
  title={Enhancer and promoter activity in the JH to IGHM intron of the Pekin duck, Anas platyrhynchos.},
  author={M-L. Lundqvist and Bryan R McElveen and Darlene L. Middleton and Gregory W. Warr},
  journal={Developmental and comparative immunology},
  year={2007},
  volume={31 3},
  pages={
          286-95
        }
}
A transcriptional enhancer, Emu, was defined in the IGH locus of the Pekin duck, Anas platyrhynchos. Regions of DNA from the JH to IGHM intron were cloned into reporter constructs containing the SV40 promoter and transiently transfected into chicken B and T lymphocytes. A strong transcriptional activity, of several hundred-fold greater than that of a reporter construct with the promoter alone, was localized to a 281bp region that contains 2 E-box motifs, CAGCTG. This fragment showed enhancer… CONTINUE READING