Enhancement of translation initiation by A/T-rich sequences downstream of the initiation codon in Escherichia coli.

@article{Qing2003EnhancementOT,
  title={Enhancement of translation initiation by A/T-rich sequences downstream of the initiation codon in Escherichia coli.},
  author={G Qing and Bing Xia and M. Inouye},
  journal={Journal of molecular microbiology and biotechnology},
  year={2003},
  volume={6 3-4},
  pages={133-44}
}
The region located downstream of the initiation codon constitutes part of the translation initiation signal, significantly affecting the level of protein expression in E. coli. In order to determine its influence on translation initiation, we inserted random 12-base sequences downstream of the initiation codon of the lacZ gene. A total of 119 random clones showing higher beta-galactosidase activities than the control lacZ gene were isolated and subsequently sequenced. Analysis of these clones… CONTINUE READING
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