Enhancement of transformation rates in higher plants by low-dose irradiation: Are DNA repair systems involved in the incorporation of exogenous DNA into the plant genome?

@article{Khler1989EnhancementOT,
  title={Enhancement of transformation rates in higher plants by low-dose irradiation: Are DNA repair systems involved in the incorporation of exogenous DNA into the plant genome?},
  author={Fabian K{\"o}hler and Greet Cardon and M A Poehlman and Ravinder Gill and Otto Schieder},
  journal={Plant Molecular Biology},
  year={1989},
  volume={12},
  pages={189-199}
}
Irradiation (X-ray; 5–15 Gy) of protoplasts treated with plasmid-DNA and PEG yielded higher transformation rates in comparison to non-irradiated protoplasts transformed by the same method. This could be demonstrated for four plant species. The irradiation doses used did not affect the total number of colonies regenerated without selection pressure, but… CONTINUE READING