Enhancement of sperm acrosin activity by glycerol-pretreatment — quantitative estimations

Acetic acid treatment of spermatozoa, a method suitable for optimal extraction of the acrosomal proteinase acrosin, was used to show the influence of glycerol on sperm acrosin activity. Short-time pretreatment of semen samples with glycerol in concentrations up to 35% (v/v) caused a 1.5–2.5 fold increase in sperm acrosin activity. Higher glycerol… CONTINUE READING