Enhancement of phenazine-1-carboxylic acid production using batch and fed-batch culture of gacA inactivated Pseudomonas sp. M18G.

Abstract

The enhancement of phenazine-1-carboxylic acid (PCA) production was investigated in a 10l fermentor using a gacA chromosomally inactivated mutant Pseudomonas sp. M18G combined with DO-stat feeing strategy. In batch culture, the optimal growth conditions with a two-stage agitation and aeration at 1.72vvm improved PCA production to 1987mg/l at 48h fermentation. A mathematical kinetic model consisting of cell growth, high PCA production, and substrate consumption was developed to simulate the batch culture process. Further increases in PCA production were achieved in a DO-stat fed-batch system which maintained a constant DO of 20% by a two-pulse glucose feeding strategy. With a total 6.6g/l glucose feeding, the maximum PCA production of 2597mg/l was obtained at 72h fermentation, resulting in a 44.5% increase in PCA production and 10-fold greater cell numbers compared to the batch culture. The combination strategies might pave a new way for the phenazine derivative commercial production using various pseudomonads.

DOI: 10.1016/j.biortech.2009.12.120

Statistics

010020020102011201220132014201520162017
Citations per Year

208 Citations

Semantic Scholar estimates that this publication has 208 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Li2010EnhancementOP, title={Enhancement of phenazine-1-carboxylic acid production using batch and fed-batch culture of gacA inactivated Pseudomonas sp. M18G.}, author={Yaqian Li and Haixia Jiang and Xilin Du and Xianqing Huang and Xuehong Zhang and Yuhong Xu and Yuquan Xu}, journal={Bioresource technology}, year={2010}, volume={101 10}, pages={3649-56} }