Enhancement of paclitaxel-induced apoptosis in HER2-overexpressing human breast cancer cells by a pertuzumab mimetic peptide, HRAP.

Abstract

HRAP, a pentapeptide designed to bind with the pertuzumab interacting site in an extracellular domain of the HER2 molecule, enhanced the cytotoxicity of paclitaxel in HER2-overexpressing human breast cancer cell lines, BT474 and SKBR-3, but not in MDA-231 cells, which express lower levels of HER2. HRAP enhanced mitochondria-dependent apoptosis induced by paclitaxel in SKBR-3 and BT-474, but not in MDA-231. HRAP enhanced the inhibition of phosphorylation of serine 473 in Akt and Ser380/Thy382/The383 in PTEN. These results suggest that HRAP enhances paclitaxel-induced apoptosis in a manner dependent on the PTEN/Akt signal transduction pathway.

DOI: 10.1016/j.jbiosc.2010.02.007

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@article{Nakajima2010EnhancementOP, title={Enhancement of paclitaxel-induced apoptosis in HER2-overexpressing human breast cancer cells by a pertuzumab mimetic peptide, HRAP.}, author={Hiroo Nakajima and Naruhiko Mizuta and Koichi Sakaguchi and Ikuya Fujiwara and Atsushi Yoshimori and Junji Magae and Sei-ichi Tanuma}, journal={Journal of bioscience and bioengineering}, year={2010}, volume={110 2}, pages={250-3} }