Enhancement of p53 sequence-specific binding by DNA supercoiling

@article{Paleek2004EnhancementOP,
  title={Enhancement of p53 sequence-specific binding by DNA supercoiling},
  author={Emil Pale{\vc}ek and V{\'a}clav Br{\'a}zda and Eva Br{\'a}zdov{\'a} Jagelsk{\'a} and Petr Pe{\vc}inka and Lenka Karlovsk{\'a} and Marie Br{\'a}zdov{\'a}},
  journal={Oncogene},
  year={2004},
  volume={23},
  pages={2119-2127}
}
Using a new competition assay, we investigated the effect of DNA negative supercoiling on the DNA sequence-specific binding (SSDB) of human wild-type (wt) p53 protein. We found that supercoiled (sc) pBluescript DNAs with different inserted p53 target sequences were stronger competitors than a mixture of scDNA pBluescript with the given 20-mer target oligodeoxynucleotide. ScDNAs were always better competitors than their linearized or relaxed forms. Two DNAs with extruded cruciforms within the… CONTINUE READING
BETA

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 15 CITATIONS

References

Publications referenced by this paper.
SHOWING 1-10 OF 29 REFERENCES

Eur

  • E Palecek, M Brazdova, +4 authors TM. Jovin
  • J. Biochem., 268, 573–581.
  • 2001
Highly Influential
4 Excerpts

Mol

  • JM Espinosa, Emerson BM.
  • Cell, 8, 57–69.
  • 2001
Highly Influential
6 Excerpts

J

  • M Stros, E. Muselikova
  • Biol. Chem., 275, 35699–35707.
  • 2000
Highly Influential
28 Excerpts

Proc

  • AK Nagaich, VB Zhurkin, SR Durell, RL Jernigan, E Appella, RE. Harrington
  • Natl. Acad. Sci. USA, 96, –1880.
  • 1999
Highly Influential
4 Excerpts

Biochem

  • V Brazda, J Palecek, S Pospisilova, B Vojtesek, E. Palecek
  • Biophys. Res. Commun., 267, 934–939.
  • 2000
Highly Influential
6 Excerpts

Cell Mol

  • F Janus, N Albrechtsen, I Dornreiter, L Wiesmuller, F Grosse, W. Deppert
  • Life Sci., 55, 12–27.
  • 1999
Highly Influential
4 Excerpts

J

  • T Gohler, M Reimann, +4 authors W. Deppert
  • Biol. Chem., 8, 8.
  • 2002

J

  • E Jagelska, V Brazda, S Pospisilova, B Vojtesek, E. Palecek
  • Immunol. Methods, 267, 227.
  • 2002

Mol

  • K McKinney, C. Prives
  • Cell. Biol., 22, 6797–6808.
  • 2002

J

  • H Zhou, Y Zhang, +4 authors RE. Harrington
  • Mol. Biol., 306, 227–238.
  • 2001

Similar Papers

Loading similar papers…