Enhancement of immunostimulatory properties of exosomal vaccines by incorporation of fusion-competent G protein of vesicular stomatitis virus.

Abstract

Exosomes have been proposed as candidates for therapeutic immunization. The present study demonstrates that incorporation of the G protein of vesicular stomatitis virus (VSV-G) into exosome-like vesicles (ELVs) enhances their uptake and induces the maturation of dendritic cells. Targeting of VSV-G and ovalbumin as a model antigen to the same ELVs increased the cross-presentation of ovalbumin via an endosomal acidification mechanism. Immunization of mice with VSV-G and ovalbumin containing ELVs led to an increased IgG2a antibody response, expansion of antigen-specific CD8 T cells, strong in vivo CTL responses, and protection from challenge with ovalbumin expressing tumor cells. Thus, incorporation of VSV-G and targeting of antigens to ELVs are attractive strategies to improve exosomal vaccines.

DOI: 10.1016/j.vaccine.2008.04.069
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@article{Temchura2008EnhancementOI, title={Enhancement of immunostimulatory properties of exosomal vaccines by incorporation of fusion-competent G protein of vesicular stomatitis virus.}, author={Vladimir Temchura and Matthias Tenbusch and Godwin W Nchinda and Ghulam Nabi and Bettina G Tippler and Maryna Zelenyuk and Oliver Wildner and Klaus Uberla and Seraphin Kuate}, journal={Vaccine}, year={2008}, volume={26 29-30}, pages={3662-72} }