Enhancement of gene targeting in human cells by intranuclear permeation of the Saccharomyces cerevisiae Rad52 protein

@inproceedings{Kalvala2010EnhancementOG,
  title={Enhancement of gene targeting in human cells by intranuclear permeation of the Saccharomyces cerevisiae Rad52 protein},
  author={Arjun Kalvala and Giuseppe Rainaldi and Cristina Di Primio and Vania Liverani and A. Falaschi and A. Galli},
  booktitle={Nucleic acids research},
  year={2010}
}
The introduction of exogenous DNA in human somatic cells results in a frequency of random integration at least 100-fold higher than gene targeting (GT), posing a seemingly insurmountable limitation for gene therapy applications. We previously reported that, in human cells, the stable over-expression of the Saccharomyces cerevisiae Rad52 gene (yRAD52), which plays the major role in yeast homologous recombination (HR), caused an up to 37-fold increase in the frequency of GT, indicating that… CONTINUE READING

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