Enhancement of Somatic Embryogenesis and Production of Developmentally Arrested Embryos in Nigella sativa L.

  title={Enhancement of Somatic Embryogenesis and Production of Developmentally Arrested Embryos in Nigella sativa L.},
  author={H. M. Elhag and Mahmoud M. El-Olemy and Mansour Sulaiman Al-Said},
Somatic embryogenesis of Nigella sativa was investigated with the objective of inducing and isolating somatic embryos for biosynthetic studies. Callus cultures were initiated from leaf, stem, and root explants of axenic seedlings on MSB5 basal medium supplemented with kinetin (0.46 µM) and 2,4-D (4.5 or 13.5 µM) or NAA (5.4 or 16.2 µM) in the dark. Cultures initiated and subcultured on medium containing NAA produced friable callus with numerous roots regardless of explant type. Cultures… Expand

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