Enhancement of Sf9 Cells and Baculovirus Production Employing Grace’s Medium Supplemented with Milk Whey Ultrafiltrate

@article{Batista2005EnhancementOS,
  title={Enhancement of Sf9 Cells and Baculovirus Production Employing Grace’s Medium Supplemented with Milk Whey Ultrafiltrate},
  author={Fabiana Regina Xavier Batista and Carlos Augusto Pereira and Ronaldo Zucatelli Mendonça and {\^A}ngela Maria Moraes},
  journal={Cytotechnology},
  year={2005},
  volume={49},
  pages={1-9}
}
Animal cells can be cultured both in basal media supplemented with fetal bovine serum (FBS) and in serum-free media. In this work, the supplementation of Grace’s medium with a set of nutrients to reduce FBS requirements in Spodoptera frugiperda (Sf9) cell culture was evaluated, aiming the production of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) at a cost lower than those for the production using Sf900 II medium. In Grace’s medium supplemented with glucose, Pluronic F68 (PF68) and yeast… CONTINUE READING