Enhancement of PCR amplification yield and specificity using AmpliTaq Gold DNA polymerase.

@article{Moretti1998EnhancementOP,
  title={Enhancement of PCR amplification yield and specificity using AmpliTaq Gold DNA polymerase.},
  author={Tamyra R Moretti and Barbara W. Koons and Bruce Budowle},
  journal={BioTechniques},
  year={1998},
  volume={25 4},
  pages={716-22}
}
Inadequate yields of PCR product and the generation of nonspecific PCR products can complicate genotyping studies, particularly when the DNA template is of inferior quality and/or has a low-copy number. In this study, the ability of AmpliTaq Gold DNA Polymerase to enhance the specificity and yield of amplification was evaluated in a quadruplex short tandem repeat (STR) system in which a nonspecific PCR product and poor yield had been previously observed with AmpliTaq DNA Polymerase usage… CONTINUE READING

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