Enhancement of PCR Amplification of Moderate GC-Containing and Highly GC-Rich DNA Sequences

@article{Strien2013EnhancementOP,
  title={Enhancement of PCR Amplification of Moderate GC-Containing and Highly GC-Rich DNA Sequences},
  author={Juliane rer. nat. Strien and Juliane Sanft and Gerhard Mall},
  journal={Molecular Biotechnology},
  year={2013},
  volume={54},
  pages={1048-1054}
}
PCR is a commonly used and highly efficient technique in biomolecular laboratories for specific amplification of DNA. However, successful DNA amplification can be very time consuming and troublesome because many factors influence PCR efficiency. Especially GC-rich DNA complicates amplification because of generation of secondary structures that hinder denaturation and primer annealing. We investigated the impact of previously recommended additives such as dimethylsulfoxide (DMSO), magnesium… CONTINUE READING
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