Enhancement of Chlorella vulgaris cell density: Shake flask and bench-top photobioreactor studies to identify and control limiting factors

  title={Enhancement of Chlorella vulgaris cell density: Shake flask and bench-top photobioreactor studies to identify and control limiting factors},
  author={Yuvraj and Ambarish Sharan Vidyarthi and Jeeoot Singh},
  journal={Korean Journal of Chemical Engineering},
Low cell density is a major bottleneck in any microalgal bioprocess that prevents the large scale exploitation of this potential bioresource from commercialization of commodities like biofuels. Control of factors limiting growth is the key to enhancing cell density. Factors limiting photoautotrophic growth of C. vulgaris were identified and controlled to a possible extent. Limiting CO2-transfer rate, light attenuation, scarcity of nutrients, and high pH compounded to retard growth gradually in… Expand

Figures and Tables from this paper

Improvements in Conventional Modeling Practices for Effective Simulation and Understanding of Microalgal Growth in Photobioreactors: an Experimental Study
This work identifies shortcomings of conventional practices in unstructured modeling of microalgal growth, thereby exploring the possibility of improving simulation of algal cultures. Light- andExpand
Bioremediation and nutrient removal from wastewater by Chlorella vulgaris
P WW, S WW and P E media were found to provide an appropriate quantity and balance of nutrients to promote significantly more rapid algal growth than the standard medium MLA, with high nutrient RE achieved at the end of cultivation period. Expand
View on a mechanistic model of Chlorella vulgaris in incubated shake flasks
Kinetic growth models are a useful tool for a better understanding of microalgal cultivation and for optimizing cultivation conditions, and growth-related model parameters were estimated by application of an extensive cross validation procedure. Expand
The conversion of carbon dioxide from biogas into phototrophic microalgal biomass
This study examines the conversion of CO2 from biogas into microalgal biomass during photosynthetic biogas upgrading. In this process, CO2 is separated from biogas by microalgae, which use the CO2Expand
Nutrient recycle from algae hydrothermal liquefaction aqueous phase through a novel selective remediation approach
Abstract Algae have received increasing interest in the past several decades as a biofuel feedstock source. However, sustainable nutrient supply has presented algal biofuels with a major obstacle inExpand


Process Engineering for High-Cell-Density Cultivation of Lipid Rich Microalgal Biomass of Chlorella sp. FC2 IITG
In the present study, process engineering strategy was applied to achieve lipid-rich biomass with high density of Chlorella sp. FC2 IITG under photoautotrophic condition. The strategy involved mediumExpand
Elemental balancing of biomass and medium composition enhances growth capacity in high-density Chlorella vulgaris cultures.
The concept of balancing the elemental composition of growth medium with biomass composition to obtain high-density cultures is introduced and the performance of high cell density photobioreactors can be significantly enhanced by proper medium design. Expand
Achieving pH control in microalgal cultures through fed-batch addition of stoichiometrically-balanced growth media
This work provides both a media formulation and a feeding strategy with a focus on nitrogen metabolism and regulation to support high-density algal culture without buffering and shows that the instability in culture pH that is observed in microalgal cultures in the absence of buffers can be overcome through alternating utilization of ammonium and nitrate. Expand
Experimental analysis and novel modeling of semi-batch photobioreactors operated with Chlorella vulgaris and fed with 100% (v/v) CO2.
Abstract In order to viably scale up the microalgae based technology for CO 2 capture and biofuels production, suitable mathematical models should be developed. In particular, since the potentialExpand
Effect of iron on growth and lipid accumulation in Chlorella vulgaris.
The economic feasibility of algal mass culture for biodiesel production is enhanced by the increase in biomass productivity and storage lipids and a simple and rapid method determining the lipid accumulation in C. vulgaris with spectrofluorimetry was developed. Expand
Systematic investigation of biomass and lipid productivity by microalgae in photobioreactors for biodiesel application.
A methodology to investigate the potential of given microalgae species for biodiesel production by characterizing their productivity in terms of both biomass and lipids found that the highest total lipid content was achieved with N. oleoabundans and Chlorella vulgaris, and these two species showed similar TAG productivities. Expand
Green microalga Chlorella vulgaris as a potential feedstock for biodiesel
BACKGROUND: A major bottleneck in microalgal biodiesel production is lipid content, which is often low in microalgal species. The present study examines Chlorella vulgaris as a potential feedstockExpand
Evolution, detrimental effects, and removal of oxygen in microalga cultures: A review
Large quantity of oxygen evolves as a byproduct of photosynthesis in microalga cultivation. As a result, dissolved oxygen of 100–400% air saturation or even higher is commonly observed in microalgalExpand
Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans
The effects of nitrogen sources and their concentrations on cell growth and lipid accumulation of Neochloris oleoabundans, one of the most promising oil-rich microalgal species, are studied. Expand
Biomass Production Potential of a Wastewater Alga Chlorella vulgaris ARC 1 under Elevated Levels of CO2 and Temperature
The results indicated that Chlorella vulgaris grew better at elevated CO2 level at 30°C, albeit with lesser efficiencies at higher temperatures, as well as differential responses of the alga, assessed in terms of NaH14CO3 uptake and carbonic anhydrase activity, to increases in temperature. Expand