Enhancement of American chestnut somatic seedling production

  title={Enhancement of American chestnut somatic seedling production},
  author={Gisele M. de Andrade and Scott A. Merkle},
  journal={Plant Cell Reports},
Somatic embryogenesis holds promise for mass propagation of American chestnut trees bred or genetically engineered for resistance to chestnut blight. However, low germination frequency of chestnut somatic embryos has limited somatic seedling production for this forest tree. We tested the effects of culture regime (semi-solid versus liquid), cold treatment, AC and somatic embryo morphology (i.e., cotyledon number) on germination and conversion of the somatic embryos. Cold treatment for 12 weeks… CONTINUE READING
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