Enhancement of Activity of la,25-Dihydroxyvitamin D3 for Growth Inhibition and Differentiation Induction of Human Myelomonocytic Leukemia Cells by Tretinoin Tocoferil, an a-Tocopherol Ester of All-trans Retinoic Acid

Abstract

Tretinoin tocoferil is an a-tocopherol ester of all-trans retinoic acid (RA) and safely used in the treatment of skin ulcer. Tretinoin tocoferil inhibited proliferation of human promyelocytic leukemia HL-60 cells and induced granulocytic differentiation of the cells, but less than RA. a-Tocopherol did not affect differentiation of HL-60 cells, but at high concentrations enhanced its nitroblue tetrazolium (NBT)-reducing activity and expression of surface antigen CD11 b, which are markers of myelomonocytic differentiation induced by RA. Tretinoin tocoferil increased NBT reduction in HL-60 cells treated with RA. It also enhanced the differentiation of HL60 cells induced by dimethyl sulfoxide, phorbol-12-myristate 13-acetate or Ia,Zfi-dihydroxyvitamin D3 (VD3 1. In combina-

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Cite this paper

@inproceedings{Makishima2002EnhancementOA, title={Enhancement of Activity of la,25-Dihydroxyvitamin D3 for Growth Inhibition and Differentiation Induction of Human Myelomonocytic Leukemia Cells by Tretinoin Tocoferil, an a-Tocopherol Ester of All-trans Retinoic Acid}, author={Makoto Makishima and Yasuhiro Kanatani and Yuri Yamamoto-Yamaguchi and Yoshio Honma}, year={2002} }