Enhancement of α-amylase production by integrating and amplifying the α-amylase gene of Bacillus amyloliquefaciens in the genome of Bacillus subtilis

Abstract

The α-amylase gene of Bacillus amyloliquefaciens was integrated into the genome of Bacillus subtilis by homologous recombination. In the first transformation step, several strains were obtained carrying the α-amylase gene as two randomly located copies. These strains produced α-amylase in the quantities comparable with that of the multicopy plasmid pKTH10… (More)
DOI: 10.1007/BF00257255

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