Enhancement and quenching of single-molecule fluorescence.

  title={Enhancement and quenching of single-molecule fluorescence.},
  author={Pascal Anger and Palash Bharadwaj and Luk{\'a}s Novotn{\'y}},
  journal={Physical review letters},
  volume={96 11},
We present an experimental and theoretical study of the fluorescence rate of a single molecule as a function of its distance to a laser-irradiated gold nanoparticle. The local field enhancement leads to an increased excitation rate whereas nonradiative energy transfer to the particle leads to a decrease of the quantum yield (quenching). Because of these competing effects, previous experiments showed either fluorescence enhancement or fluorescence quenching. By varying the distance between… 

Figures from this paper

Nanoplasmonic enhancement of single-molecule fluorescence

We demonstrate that the fluorescence rate from a single molecule with near-unity quantum yield can be enhanced by a factor of ≈10 by use of a single laser-irradiated noble metal nanoparticle. The

Spectral dependence of single molecule fluorescence enhancement.

The spectral tunability of this antenna effect is demonstrated and it is shown that maximum enhancement is achieved when the emission frequency is red-shifted from the surface plasmon resonance of the particle.

Emission and excitation contributions to enhanced single molecule fluorescence by gold nanometric apertures.

A broad range of nanoaperture diameters are characterized from 80 to 310 nm, and the link between the fluorescence enhancement and the local photonic density of states is highlighted, to increase the effectiveness of fluorescence-based single molecule detection.

Fluorescence enhancement of quantum emitters with different energy systems near a single spherical metal nanoparticle.

The numerical results provide the first theoretical prediction showing that the MNP may selectively enhance a certain fluorescence process among various ones.

Spectral variation of fluorescence lifetime near single metal nanoparticles

The explicit comparison between experiment and simulation offers an insight into the spectral engineering of LSP mediated fluorescence and may lead to optimized application in sensing and biomedicine.

Identification of molecules through the fluorescence enhancement by a metal tip

A fluorescence enhancement phenomenon, which is realized as a result of a sharp increase in the radiative decay rate of a quantum dipole emitter (QDE) is investigated theoretically in the vicinity of

Plasmon-enhanced fluorescence intensities and rates permit super-resolution imaging of enhanced local fields

Single-molecule fluorescence is a powerful tool for imaging structures below the standard diffraction limit of light. The resolution gain comes from fitting the emission of isolated fluorophores, and

Distance dependence of near-field fluorescence enhancement and quenching of single quantum dots

A combined total internal reflection fluorescence microscopy–AFM setup is used and the influence of interfering processes such as field enhancement confined at interface boundaries, mirror dipoles and (resonant) dipolar coupling is revealed and quantified.

Plasmonic Enhancement of Single-Molecule Fluorescence Near a Silver Nanoparticle

It is found that on average the Cy5 molecules bound to metal nanoparticles are approximately 15-fold brighter than that of free dyes, and that single molecule lifetimes are shorter as compared to free fluorophores.

Reversible polarization control of single photon emission.

Reversible and a-priori control of the polarization of a photon emitted by a single molecule is presented by introducing a nanoscale metal object in its near field by using the multiple multipole method.

Single-molecule spontaneous emission close to absorbing nanostructures

The spontaneous emission of a single molecule is substantially modified close to a metallic nanostructure. We study the spectral behavior of the radiative and nonradiative decay rates and of the

Single-molecule near-field optical energy transfer microscopy with dielectric tips.

The experiment provides surprising new insights into the interactions between a fluorescent molecule and a dielectric tip and holds promise for applications in ultra high-resolution near-field optical imaging at the level of single fluorophores.

Fluorescence quenching in tip-enhanced nonlinear optical microscopy

We describe the theoretical treatment of fluorescence quenching in tip-enhanced nonlinear optical microscopy (TENOM). Finite difference time domain simulations demonstrate that while sharp pyramidal

High-resolution imaging of single fluorescent molecules with the optical near-field of a metal tip.

We show that a concentration of light at a metal tip allows near-field optical imaging of single fluorescent dye molecules at very high resolution, despite strong quenching effects. Details as small

Optical near-field enhancement at a metal tip probed by a single fluorophore

The optical near-field in the vicinity of a metal tip is mapped using a single-molecule optical probe. We observe an enhancement of the fluorescence signal by a factor of 5.7±0.3, clearly larger than

Surface-enhanced emission from single semiconductor nanocrystals.

The enhanced excited state decay process for NCs coupled to rough metal substrates effectively competes with the Auger relaxation process, allowing us to observe both charged and neutral exciton emission from these NC quantum dots.

Gold nanoparticles quench fluorescence by phase induced radiative rate suppression.

The fluorescence quantum yield of Cy5 molecules attached to gold nanoparticles via ss DNA spacers via ssDNA spacers is measured for Cy5-nanoparticle distances between 2 and 16 nm to show distance dependent quantum efficiency.

Single molecule emission characteristics in near-field microscopy.

The 2D computation reveals insight into the lifetime behaviors and provides guidance for nonperturbative spectroscopic measurements with NSOM, capable of predicting molecular emission properties in front of a metal/dielectric interface of arbitrary geometry.

Measuring the quantum efficiency of the optical emission of single radiating dipoles using a scanning mirror.

Using scanning probe techniques, controlled manipulation of the radiation from single dipoles is shown and comparison of data with theoretical models allows determination of the quantum efficiency of each radiating dipole.

Single molecule fluorescence in inhomogeneous environments

A single molecule in an inhomogeneous environment is studied theoretically using phenomenological theory. The molecule is located on a planar glass substrate and a finite sized, three dimensional