Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene with Bcl-xL.

@article{Zustiak2014EnhancedTR,
  title={Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene with Bcl-xL.},
  author={Matthew P Zustiak and Lisa Jose and Yueqing Xie and Jianwei Zhu and Michael J. Betenbaugh},
  journal={Biotechnology journal},
  year={2014},
  volume={9 9},
  pages={1164-74}
}
Transient gene expression is gaining popularity as a method to rapidly produce recombinant proteins in mammalian cells. Although significant improvements have been made, in terms of expression, more improvements are needed to compete with the yields achievable in stable gene expression. Much progress has come from optimization of transfection media and parameters, as well as altering culturing conditions to enhance productivity. Recent studies have included cell lines engineered for apoptosis… CONTINUE READING

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