Enhanced transgene expression in cord blood CD34(+)-derived hematopoietic cells, including developing T cells and NOD/SCID mouse repopulating cells, following transduction with modified trip lentiviral vectors.

@article{Sirven2001EnhancedTE,
  title={Enhanced transgene expression in cord blood CD34(+)-derived hematopoietic cells, including developing T cells and NOD/SCID mouse repopulating cells, following transduction with modified trip lentiviral vectors.},
  author={A Sirven and Emmanuel Ravet and Pierre Charneau and V{\'e}ronique Zennou and Laure Coulombel and Denise Gu{\'e}tard and Francoise Pflumio and Anne Dubart-Kupperschmitt},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2001},
  volume={3 4},
  pages={438-48}
}
The recent development of lentivirus-derived vectors is an important breakthrough in gene transfer technology because these vectors allow transduction of nondividing cells such as hematopoietic stem cells (HSC), due to an active nuclear import of reverse-transcribed vector DNA. We recently demonstrated that addition of the central DNA flap of HIV-1 to an HIV-derived lentiviral vector strikingly increases transduction of CD34(+) cells. We now describe improvements of the transduction protocol… CONTINUE READING

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