Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase.

@article{Amitai2006EnhancedSH,
  title={Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase.},
  author={Gabriel Amitai and Leonid Gaidukov and Rellie Adani and Shelly Yishay and Guy Yacov and Moshe Kushnir and Shai Teitlboim and Michal Lindenbaum and Peter Bel and Olga Khersonsky and Dan S. Tawfik and Haim Meshulam},
  journal={The FEBS journal},
  year={2006},
  volume={273 9},
  pages={1906-19}
}
We addressed the ability of various organophosphorus (OP) hydrolases to catalytically scavenge toxic OP nerve agents. Mammalian paraoxonase (PON1) was found to be more active than Pseudomonas diminuta OP hydrolase (OPH) and squid O,O-di-isopropyl fluorophosphatase (DFPase) in detoxifying cyclosarin (O-cyclohexyl methylphosphonofluoridate) and soman (O-pinacolyl methylphosphonofluoridate). Subsequently, nine directly evolved PON1 variants, selected for increased hydrolytic rates with a… CONTINUE READING
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