Enhanced spectral resolution in immobilized peptides and proteins by combining chemical shift sum and difference spectroscopy.

@article{Luca2002EnhancedSR,
  title={Enhanced spectral resolution in immobilized peptides and proteins by combining chemical shift sum and difference spectroscopy.},
  author={Sorin Luca and Marc Baldus},
  journal={Journal of magnetic resonance},
  year={2002},
  volume={159 2},
  pages={
          243-9
        }
}
A two-dimensional correlation experiment is introduced that records the sum and difference chemical shift of two scalar or dipolar coupled nuclei. Statistical results indicate that the suggested pulse scheme can significantly increase the possibility of separating chemical shift contributions due to residue type and backbone conformation in immobilized peptides and proteins. Experimental applications demonstrate the theoretical concept and lead to the predicted resolution enhancement between… CONTINUE READING

Figures, Tables, and Topics from this paper.

Explore Further: Topics Discussed in This Paper

References

Publications referenced by this paper.
SHOWING 1-10 OF 51 REFERENCES

B

J. Pauli, M. Baldus
  • van Rossum, H. de Groot, H. Oschkinat, Backbone and side-chain C-13 and N-15 signal assignments of the alpha-spectrin SH3 domain by magic angle spinning solid-state NMR at 17.6 tesla, Chembiochem 2
  • 2001

Backbone and side - chain C13 and N15 signal assignments of the alphaspectrin SH 3 domain by magic angle spinning solid - state NMR at 17 . 6 tesla

M. Baldus J. Pauli, B. van Rossum, H. de Groot, H. Oschkinat
  • Chembiochem
  • 2001

Case , Use of chemical shifts in macromolecular structure determination

D. A. D. S. Wishart
  • Methods Enzymol .
  • 2001

Heteronuclear 2 Dcorrelations in a uniformly [ C13 , N15 ] labeled membraneprotein complex at ultra - high magnetic fields

J. Hollander T. A. Egorova-Zachernyuk, N. Fraser, +4 authors M. Baldus
  • J . Biomol . NMR
  • 2001

R

T. A. Egorova-Zachernyuk, J. Hollander, N. Fraser, P. Gast, A. J. Hoff
  • Cogdell, H.J.M. de Groot, M. Baldus, Heteronuclear 2Dcorrelations in a uniformly [C-13, N-15] labeled membraneprotein complex at ultra-high magnetic fields, J. Biomol. NMR 19
  • 2001
VIEW 1 EXCERPT