Enhanced proteome profiling by inhibiting proteolysis with small heat shock proteins.

@article{Han2005EnhancedPP,
  title={Enhanced proteome profiling by inhibiting proteolysis with small heat shock proteins.},
  author={M. Han and Jeong Wook Lee and Sang Yup Lee},
  journal={Journal of proteome research},
  year={2005},
  volume={4 6},
  pages={2429-34}
}
Proteolytic degradation is one of the critical problems in two-dimensional electrophoresis (2-DE). Here we report that small heat shock proteins (sHsps), including IbpA(Ec) and IbpB(Ec) from Escherichia coli and Hsp26(Sc) from Saccharomyces cerevisiae, are able to protect proteins in vitro from proteolytic degradation. Addition of sHsps during 2-DE of human serum or whole cell extracts of E. coli, Mannheimia succinciproducens, Arabidopsis thaliana, and human kidney cells allowed detection of up… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

Similar Papers

Loading similar papers…