Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis

@article{Hong2010EnhancedPO,
  title={Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis},
  author={Won-kyung Hong and Chul-Ho Kim and Sun-Yeon Heo and Lian Hua Luo and Baek-Rock Oh and J W Seo},
  journal={Biotechnology Letters},
  year={2010},
  volume={32},
  pages={1077-1082}
}
To improve production of ethanol from glycerol, the methylotrophic yeast Hansenula polymorpha was engineered to express the pdc and adhB genes encoding pyruvate decarboxylase and aldehyde dehydrogenase II from Zymomonas mobilis, respectively, under the control of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. The ethanol yield was 3.3-fold higher (2.74 g l−1) in the engineered yeast compared with the parent strain (0.83 g l−1). Further engineering to stimulate glycerol… CONTINUE READING