Enhanced polyploidy by glutaraldehyde in cultured HL60 leukemic cells.

@article{Sentein1986EnhancedPB,
  title={Enhanced polyploidy by glutaraldehyde in cultured HL60 leukemic cells.},
  author={Paul Sentein and Dominique Le Coq},
  journal={Leukemia research},
  year={1986},
  volume={10 5},
  pages={575-84}
}
Glutaraldehyde 10(-4) M weakened cell proliferation of HL60 cultured cells and enhanced the appearance of giant polyploid cells, up to 32.5% after 6 days. The size and structure of these cells, the quantitative changes in their DNA content with respect to diploid ones demonstrate their polyploid nature, which may be corrected by the occurrence of pluripolar mitoses. However the slowing down of cell proliferation is not enough to orient the cells towards differentiation. Maturation of polyploid… CONTINUE READING