Enhanced oxygen delivery reverses anaerobic metabolic states in prolonged sandwich rat hepatocyte culture.

@article{Bader1999EnhancedOD,
  title={Enhanced oxygen delivery reverses anaerobic metabolic states in prolonged sandwich rat hepatocyte culture.},
  author={Augustinus Bader and Norbert Fr{\"u}hauf and Markus Tiedge and M Drinkgern and Loredana De Bartolo and J T Borlak and Gustav Steinhoff and Axel Haverich},
  journal={Experimental cell research},
  year={1999},
  volume={246 1},
  pages={221-32}
}
It must be assumed that current petri dish primary hepatocyte culture models do not supply sufficient amounts of oxygen and thus cause anaerobic metabolism of the cells. This is contrary to the physiologic state of the cells. In vivo the liver is a highly vascularized organ with a rather high blood flow rate of a mixture of arterial and venous blood. The aim of the present study was to show the oxygen dependence of primary rat hepatocytes in long-term culture and to define appropriate… CONTINUE READING

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