Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034

  title={Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034},
  author={Luciana G. Ruiz Rodr{\'i}guez and Kadri Aller and Elena Bru and Luc De Vuyst and Elvira Maria Hebert and Fernanda Mozzi},
  journal={Applied Microbiology and Biotechnology},
Mannitol is a natural low-calorie sugar alcohol produced by certain (micro)organisms applicable in foods for diabetics due to its zero glycemic index. In this work, we evaluated mannitol production and yield by the fruit origin strain Fructobacillus tropaeoli CRL 2034 using response surface methodology with central composite design (CCD) as optimization strategy. The effect of the total saccharide (glucose + fructose, 1:2) content (TSC) in the medium (75, 100, 150, 200, and 225 g/l) and… 

Exploring the Genome of Fructobacillus tropaeoli CRL 2034, a Fig-Origin Strain that Produces High Levels of Mannitol from Fructose

The draft genome sequence of Fructobacillus tropaeoli CRL 2034, a strain isolated from ripe fig in Tucumán province, Argentina, could be useful to further elucidate regulatory processes of mannitol and other bioactive metabolites and to highlight the biotechnological potential of this fruit-origin FructOBacillus strain.

Fructose-rich niches traced the evolution of lactic acid bacteria toward fructophilic species

The wide frequency of isolation of FLAB from fructose feeding insects has been deepened to reveal their ecological significance, and findings to date acquired are consistent with a metabolic model in which FLAB display a reliance on environmental niches and the degree of host specificity.

Evaluation of lactic acid bacteria strains isolated from fructose-rich environments for their mannitol-production and milk-gelation abilities.

It is shown that fructose-rich niches can be considered important sources of fructophilic LAB strains, with the potential to be used as starter cultures or adjunct cultures for the manufacture of mannitol-enriched fermented dairy products and beverages.

Fructophilic Lactic Acid Bacteria, a Unique Group of Fructose-Fermenting Microbes

FLAB have significantly fewer genes for carbohydrate metabolism than other LAB, especially due to the lack of complete phosphotransferase system (PTS) transporters.

Diversity and Functional Properties of Lactic Acid Bacteria Isolated From Wild Fruits and Flowers Present in Northern Argentina

The isolated fruit- and flower-origin LAB displayed functional properties that validate their potential use in the manufacture of fermented fruit-based products setting the background for the design of novel functional foods.


The goal of this work was to summarize the important physicochemical properties, mechanism of action, production, applications, incompatibilities, polymorphism, and particularly the analytical methodologies published in the last five decades for quantification.

Interactive analysis of biosurfactants in fruit-waste fermentation samples using BioSurfDB and MEGAN

Fruit waste is suggested to be a promising substrate for fermentative processes because the associated microbiota may be able to produce biosurfactants that are potentially useful for the agricultural, chemical, food and pharmaceutical industries.

Are fructophilic lactic acid bacteria (FLAB) beneficial to humans?

The possible role FLAB may have in the human gastro-intestinal tract (GIT) is discussed and the advantages and disadvantages these bacteria might have in individuals with a diet high in fructose are highlighted.



Response Surface Optimization of Exopolysaccharide Production from Sugarcane Juice by Lactobacillus confusus TISTR 1498

Lactobacillus confusus TISTR 1498, isolated from Thai traditional fermented pork (Nham), could secrete large amounts of exopolysaccharides (EPS). Response surface methodology was applied to optimize

Efficient mannitol production by wild-type Lactobacillus reuteri CRL 1101 is attained at constant pH using a simplified culture medium

The mannitol production obtained in this study is the highest reported so far by a wild-type L. reuteri strain and, more interestingly, using a simplified culture medium.

Lactobacillus reuteri CRL 1101 highly produces mannitol from sugarcane molasses as carbon source

Mannitol production by Lactobacillus reuteri CRL 1101 was evaluated using sugarcane molasses as low-cost energy source and successfully produced mannitol from sugarcanes molasses being a promising candidate for microbial mann Capitolitol synthesis using low- cost substrate.

Global Analysis of Mannitol 2-Dehydrogenase in Lactobacillus reuteri CRL 1101 during Mannitol Production through Enzymatic, Genetic and Proteomic Approaches

Deep insight is offered into the polyol formation by a Lactobacillus strain with biotechnological potential in the nutraceutics and pharmaceutical areas by observing that MDH was markedly induced by the presence of fructose.

Mannitol production by heterofermentative Lactobacillus reuteri CRL 1101 and Lactobacillus fermentum CRL 573 in free and controlled pH batch fermentations

Mannitol production and yields were higher under constant pH conditions compared with fermentations with free pH, the increase being more pronounced in the case of the L. fermentum strain.

Effect of salt nutrients on mannitol production by Lactobacillus intermedius NRRL B-3693

  • B. Saha
  • Chemistry, Medicine
    Journal of Industrial Microbiology and Biotechnology
  • 2006
The effects of four salt nutrients on the production of mannitol by Lactobacillus intermedius NRRL B-3693 in a simplified medium containing 300 g fructose, 5 g soy peptone, and 50 g corn steep liquor per liter in pH-controlled fermentation at 5.0 at 37°C were evaluated using a fractional factorial design.

Biotechnological and in situ food production of polyols by lactic acid bacteria

This review deals with the spontaneous formation of mannitol and erythritol in fermented foods and their biotechnological production by heterofermentative LAB and briefly presented the metabolic engineering processes applied for polyol formation.