Enhanced indirubin production in recombinant Escherichia coli harboring a flavin-containing monooxygenase gene by cysteine supplementation.

@article{Han2012EnhancedIP,
  title={Enhanced indirubin production in recombinant Escherichia coli harboring a flavin-containing monooxygenase gene by cysteine supplementation.},
  author={G. Han and G. Gim and Wonduck Kim and Sun-il Seo and S. Kim},
  journal={Journal of biotechnology},
  year={2012},
  volume={164 2},
  pages={
          179-87
        }
}
  • G. Han, G. Gim, +2 authors S. Kim
  • Published 2012
  • Chemistry, Medicine
  • Journal of biotechnology
In our previous study, a batch fermentation of recombinant Escherichia coli DH5α cells harboring the fmo gene from Methylophaga aminisulfidivorans MP(T) produced indirubin (5.0mg/L) and indigo (920mg/L) in a 5L fermenter containing tryptophan medium (2g/L tryptophan, 5g/L yeast extract, 10g/L NaCl). In this study, it was found that indirubin production greatly increased when 0.36g/L cysteine was added to the tryptophan medium, although cysteine inhibited the growth of the recombinant E. coli… Expand
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References

SHOWING 1-10 OF 35 REFERENCES
Application of metabolic engineering to improve both the production and use of biotech indigo
A novel flavin-containing monooxygenase from Methylophaga sp strain SK1 and its indigo synthesis in Escherichia coli.
Protein engineering of toluene ortho-monooxygenase of Burkholderia cepacia G4 for regiospecific hydroxylation of indole to form various indigoid compounds
...
1
2
3
4
...