Enhanced immunization techniques to obtain highly specific monoclonal antibodies

  title={Enhanced immunization techniques to obtain highly specific monoclonal antibodies},
  author={Rodrigo de Almeida and Cec{\'i}lia Naomi Nakamura and Marina de Lima Fontes and Elenice Deffune and S{\'e}rgio Lu{\'i}s Felisbino and Ramon Kaneno and Wagner Jos{\'e} F{\'a}varo and Athanase Billis and Marcel Ot{\'a}vio Cerri and Ana Marisa Fusco-Almeida and Maria Jos{\'e} Soares Mendes Giannini and Andrei Moroz},
  pages={46 - 54}
Despite fast advances in genomics and proteomics, monoclonal antibodies (mAbs) are still a valuable tool for areas such as the evolution of basic research in stem cells and cancer, for immunophenotyping cell populations, diagnosing and prognosis of diseases, and for immunotherapy. [] Key Method To summarize different subtractive immunization approaches successfully used for the production of highly specific antibodies, we identified scientific articles in NCBI PubMed using the following search terms…
Generation and characterization of a high‐affinity chimeric anti‐OX40 antibody with potent antitumor activity
This paper systematically investigates the antigen‐specific immune response, the number of binders, epitope bins, and functional activities of antibodies among different immunization strategies, and found that different immunizations strategies affect the biological activity of monoclonal antibodies.
CpG-Containing Oligodeoxynucleotides and Freund Adjuvant in Combination with Alum Augment the Production of Monoclonal Antibodies Against Recombinant HBsAg
CpG and Freund adjuvants could be efficient partners for alum in the immunization period of the process of monoclonal antibody production, and were found to have the highest concentration of antibody production in serum and hybridoma supernatants as well as positive clones.
Monoclonal Antibody Against Antigens of Leishmania infantum: Optimize the Growth Condition of Monoclonal Antibody-producing Hybrids
It seems in the near future, this SCCSM can be used as a growth factor for cancerous and non-cancerous cells in research centers at a wider level as it seemed that SSMCC is rich of growth factors.
Optimization of Recombinant Flavivirus Antigens for Infection Serology: Towards Syndrome-Based Multiplex Tests
Sensitive and specific pathogen detection is an essential prerequisite for the prevention and treatment of infectious diseases. The similarities of clinical symptoms and serological cross-reactivity
Cancer Immunotherapy
This chapter aims to discuss the mechanism and different types of immunotherapies used for different cancers, and to focus on recombinant products including immunostimulants, immunotoxins, antibodies, fusion proteins, engineered cytotoxic T cells, engineered immunocytokines, vaccines, checkpoint inhibitors, CAR T-cell therapy, and nanomedicine.
PEPOP 2.0: new approaches to mimic non-continuous epitopes
Original methods are reported that show how PEPOP 2.0 can assist biologists in dealing with discontinuous epitopes.
Multiple Tolerization Subtractive Immunization (MTSI) Protocol: Effects on Mice and Monoclonal Antibody Specificity
MTSI technique was successful in generating mAbs that recognize tumor-specific antigens, but not from their non-tumor counterparts, as shown in western blotting and immunofluorescence assays.
Immunochemistry-Based Diagnosis of Extrapulmonary Tuberculosis: A Strategy for Large-Scale Production of MPT64-Antibodies for Use in the MPT64 Antigen Detection Test
It is suggested that it possible to reproduce a large-volume functional polyclonal antibody with stable performance of the MPT64 test, thereby securing stable supplies and reproducibility of the test, albeit further validation remains to be done.


Targeting the proteome/epitome, implementation of subtractive immunization.
Subtractive immunization yields monoclonal antibodies that specifically inhibit metastasis
Data demonstrate that the S.I. protocol can be used for the development of unique mAbs that are reactive with antigenic determinants whose expression is elevated on metastatic human tumor cells and which function mechanistically in the metastatic cascade.
Subtractive immunization: a tool for the generation of discriminatory antibodies to proteins of similar sequence.
Monoclonal Antibody Identifies a Distinctive Epitope Expressed by Human Multiple Myeloma Cells
Monoclonal antibody VAC69 triggered cancer-specific cytotoxicity in vitro as well as in vivo using a sever combined immunodeficiency model transplanted with human MM, indicating its potential use for therapeutic intervention in MM by delivering drugs into cancer cells.
Comparison of two techniques for targeting the production of monoclonal antibodies against particular antigens.
Tolerization as a tool for generating novel monoclonal antibodies
A number of strategies are detailed using either complex protein mixtures or purified proteins as tolerogens and subsequent immunization with a closely related immunogen to recovery of high‐specificity reagents that have previously proved elusive.
Tolerization of adult mice to immunodominant proteins before monoclonal antibody production.
Use of neonatal tolerization and chemical immunosuppression for the production of monoclonal antibodies to maturation-specific sperm surface molecules.
Use of the tolerization/immunosuppression protocol has provided Mab tools to assist in the study of sperm maturation during epididymal transit, and five Mabs described also recognize cauda fluid antigens, suggesting that the maturational changes on the sperm plasma membranes arise from interactions with the epiddymal fluid.