Enhanced immunization techniques to obtain highly specific monoclonal antibodies

@article{deAlmeida2018EnhancedIT,
  title={Enhanced immunization techniques to obtain highly specific monoclonal antibodies},
  author={Rodrigo de Almeida and Cec{\'i}lia Naomi Nakamura and Marina de Lima Fontes and Elenice Deffune and S{\'e}rgio Lu{\'i}s Felisbino and Ramon Kaneno and Wagner Jos{\'e} F{\'a}varo and Athanase Billis and Marcel Ot{\'a}vio Cerri and Ana Marisa Fusco-Almeida and Maria Jos{\'e} Soares Mendes Giannini and Andrei Moroz},
  journal={mAbs},
  year={2018},
  volume={10},
  pages={46 - 54}
}
Despite fast advances in genomics and proteomics, monoclonal antibodies (mAbs) are still a valuable tool for areas such as the evolution of basic research in stem cells and cancer, for immunophenotyping cell populations, diagnosing and prognosis of diseases, and for immunotherapy. [] Key Method To summarize different subtractive immunization approaches successfully used for the production of highly specific antibodies, we identified scientific articles in NCBI PubMed using the following search terms…
Generation and characterization of a high‐affinity chimeric anti‐OX40 antibody with potent antitumor activity
TLDR
This paper systematically investigates the antigen‐specific immune response, the number of binders, epitope bins, and functional activities of antibodies among different immunization strategies, and found that different immunizations strategies affect the biological activity of monoclonal antibodies.
CpG-Containing Oligodeoxynucleotides and Freund Adjuvant in Combination with Alum Augment the Production of Monoclonal Antibodies Against Recombinant HBsAg
TLDR
CpG and Freund adjuvants could be efficient partners for alum in the immunization period of the process of monoclonal antibody production, and were found to have the highest concentration of antibody production in serum and hybridoma supernatants as well as positive clones.
Monoclonal Antibody Against Antigens of Leishmania infantum: Optimize the Growth Condition of Monoclonal Antibody-producing Hybrids
TLDR
It seems in the near future, this SCCSM can be used as a growth factor for cancerous and non-cancerous cells in research centers at a wider level as it seemed that SSMCC is rich of growth factors.
Optimization of Recombinant Flavivirus Antigens for Infection Serology: Towards Syndrome-Based Multiplex Tests
Sensitive and specific pathogen detection is an essential prerequisite for the prevention and treatment of infectious diseases. The similarities of clinical symptoms and serological cross-reactivity
Cancer Immunotherapy
TLDR
This chapter aims to discuss the mechanism and different types of immunotherapies used for different cancers, and to focus on recombinant products including immunostimulants, immunotoxins, antibodies, fusion proteins, engineered cytotoxic T cells, engineered immunocytokines, vaccines, checkpoint inhibitors, CAR T-cell therapy, and nanomedicine.
PEPOP 2.0: new approaches to mimic non-continuous epitopes
TLDR
Original methods are reported that show how PEPOP 2.0 can assist biologists in dealing with discontinuous epitopes.
Multiple Tolerization Subtractive Immunization (MTSI) Protocol: Effects on Mice and Monoclonal Antibody Specificity
TLDR
MTSI technique was successful in generating mAbs that recognize tumor-specific antigens, but not from their non-tumor counterparts, as shown in western blotting and immunofluorescence assays.
Immunochemistry-Based Diagnosis of Extrapulmonary Tuberculosis: A Strategy for Large-Scale Production of MPT64-Antibodies for Use in the MPT64 Antigen Detection Test
TLDR
It is suggested that it possible to reproduce a large-volume functional polyclonal antibody with stable performance of the MPT64 test, thereby securing stable supplies and reproducibility of the test, albeit further validation remains to be done.

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