Enhanced hydantoinase and N-carbamoylase activity on immobilisation of Agrobacterium tumefaciens

Abstract

Cell extracts of Agrobacterium tumefaciens, immobilised in calcium alginate beads, had a 7-fold increase in N-carbamoylase (N-carbamylamino acid amidohydrolase E.C. 3.5.1) activity on reaction with N-carbamylglycine. The hydantoinase (dihydropyrimidinase E.C. 3.5.2.2) and N-carbamoylase activities remained stable over 4 weeks storage at 4 °C relative to the non-immobilised enzymes, with the hydantoinase activity showing a 5-fold increase in activity relative to the non-immobilised hydantoinase. The pH optima of the immobilised hydantoinase and N-carbamoylase enzymes decreased to pH 7 and pH 8, respectively. The temperature optimum remained at 40 °C for the N-carbamoylase enzyme while the hydantoinase activity was optimal at 50 °C.

DOI: 10.1023/A:1021798602499

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Cite this paper

@article{Foster2003EnhancedHA, title={Enhanced hydantoinase and N-carbamoylase activity on immobilisation of Agrobacterium tumefaciens}, author={Ingrid M. Foster and Rosemary D. Dorrington and Stephanie Gail Burton}, journal={Biotechnology Letters}, year={2003}, volume={25}, pages={67-72} }