Enhanced hyaluronic acid production in Bacillus subtilis by coexpressing bacterial hemoglobin.

@article{Chien2007EnhancedHA,
  title={Enhanced hyaluronic acid production in Bacillus subtilis by coexpressing bacterial hemoglobin.},
  author={L. Y. Chien and Cheng-Kang Lee},
  journal={Biotechnology progress},
  year={2007},
  volume={23 5},
  pages={1017-22}
}
Bacillus subtilis strains that can produce hyaluronic acid (HA) were constructed by integrating the HA synthase gene (hasA) and the UDP-glucose dehydrogenase gene of group C Streptococcus (hasB) or of B. subtilis itself (tauD) into the amyE locus of the B. subtilis chromosome. All of the inserted genes were under the control of a strong constitutive vegII promoter of B. subtilis. Although HA production could be achieved by expressing hasA alone, coexpressing hasB or tauD with hasA could enhance… CONTINUE READING