Enhanced gene expression in insect cells and silkworm larva by modified polyhedrin promoter using repeated Burst sequence and very late transcriptional factor-1.

@article{Manohar2010EnhancedGE,
  title={Enhanced gene expression in insect cells and silkworm larva by modified polyhedrin promoter using repeated Burst sequence and very late transcriptional factor-1.},
  author={Suganthi Lavender Manohar and Shin Kanamasa and Takuya Nishina and Tatsuya Kato and Enoch Y. Park},
  journal={Biotechnology and bioengineering},
  year={2010},
  volume={107 6},
  pages={
          909-16
        }
}
The Burst of expression from polyhedrin (polh) promoter during very late phase of baculovirus infection requires a sequence located between TAAG and the translation initiation site, typically referred to as burst sequence (BS). The expression of polh promoter is stimulated by specifically binding of very late transcriptional factor 1 (VLF-1) to BS. In order to enhance the production of recombinant proteins the polh promoter was modified via a multiple BS bacmid system in which the number of BSs… CONTINUE READING

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-8 OF 8 CITATIONS