Enhanced gene detection assays for fumarate-adding enzymes allow uncovering of anaerobic hydrocarbon degraders in terrestrial and marine systems.

@article{Netzer2013EnhancedGD,
  title={Enhanced gene detection assays for fumarate-adding enzymes allow uncovering of anaerobic hydrocarbon degraders in terrestrial and marine systems.},
  author={Frederick von Netzer and Giovanni Pilloni and Sara Kleindienst and Martin Krueger and Katrin Knittel and Friederike Gr{\"u}ndger and Tillmann Lueders},
  journal={Applied and environmental microbiology},
  year={2013},
  volume={79 2},
  pages={543-52}
}
The detection of anaerobic hydrocarbon degrader populations via catabolic gene markers is important for the understanding of processes at contaminated sites. Fumarate-adding enzymes (FAEs; i.e., benzylsuccinate and alkylsuccinate synthases) have already been established as specific functional marker genes for anaerobic hydrocarbon degraders. Several recent studies based on pure cultures and laboratory enrichments have shown the existence of new and deeply branching FAE gene lineages, such as… CONTINUE READING

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