Enhanced flexibility and aphidicolin-induced DNA breaks near mammalian replication origins: implications for replicon mapping and chromosome fragility.

@article{Toledo2000EnhancedFA,
  title={Enhanced flexibility and aphidicolin-induced DNA breaks near mammalian replication origins: implications for replicon mapping and chromosome fragility.},
  author={Franck Toledo and Arnaud Coquelle and E. Yu. Svetlova and Michelle Debatisse},
  journal={Nucleic acids research},
  year={2000},
  volume={28 23},
  pages={4805-13}
}
Common fragile sites are chromosomal loci prone to breakage and rearrangement that can be induced by aphidicolin, an inhibitor of DNA polymerases. Within these loci, sites of preferential DNA breaks were proposed to correlate with peaks of enhanced DNA flexibility, the function of which remains elusive. Here we show that mammalian DNA replication origins are enriched in peaks of enhanced flexibility. This finding suggests that the search for these features may help in the mapping of replication… CONTINUE READING