Enhanced expression of type I receptors for bone morphogenetic proteins during bone formation

  title={Enhanced expression of type I receptors for bone morphogenetic proteins during bone formation},
  author={Yasuhiro Ishidou and Isao Kitajima and Hiroya Obama and Ikuro Maruyama and Fusayoshi Murata and Takeshi Imamura and N. Yamada and Peter Ten Duke and Kohei Miyazono and Takashi Sakou},
  journal={Journal of Bone and Mineral Research},
Type I receptors for bone morphogenetic proteins (BMPs), i.e., BMPR‐IA and BMPR‐IB, are transmembrane serine/threonine kinases, that bind osteogenic protein‐1 (OP‐1, also termed BMP‐7) and BMP‐4. Using antibodies specific to BMPR‐IA and ‐IB, we have studied the expression of BMP type I receptors in the bone formation process during embryonic development and fracture healing. In the mouse embryo, both BMPR‐IA and ‐IB were expressed in condensing mesenchymal cells at 13.5 days post coitum (p.c… 

The biological function of type I receptors of bone morphogenetic protein in bone

This review focuses on all three types of BMPRI, which consist of activin-like kinase 2 (ALK2, also called type IA activin receptor), activin/threonine kinase 3 (ALK3,Also called BMPRIA), and Activin- like kinase 6 (ALK6), and the current progress regarding the roles of these receptors during myogenesis, chondrogenesis, and osteogenesis.

Distinct roles of type I bone morphogenetic protein receptors in the formation and differentiation of cartilage.

It is demonstrated that the two type I BMPRs, BM PR-IA and BMPR-IB, regulate distinct processes during chick limb development and regulates apoptosis.

Bone morphogenetic protein receptor signaling is necessary for normal murine postnatal bone formation

The effects of alterations in BMP receptor function targeted to the osteoblast lineage are shown and a necessary role of B MP receptor signaling in postnatal bone growth and bone formation in vivo is demonstrated.

BMP signaling components are expressed in human fracture callus.

Up‐regulation of bone morphogenetic protein receptor IB by growth factors enhances BMP‐2‐induced human bone cell functions

Examination of the effects of TGF‐β1, FGF‐2, and PDGF‐AB on BMPR expression and BMP‐2‐mediated osteoblast functions in primary human bone cells suggests that they might positively modulate bone formation by up‐regulating BMPR‐IB in vivo.

Expression of Bone Morphogenetic Protein-6 and Bone Morphogenetic Protein Receptors in Myoepithelial Cells of Canine Mammary Gland Tumors

Findings suggest that the expression of BMP and its receptors on the myoepithelial cells might play a role in the ectopic cartilage formation in canine mammary gland tumors, especially in benign mixed tumors.

Bone morphogenetic protein receptors and their nuclear effectors in bone formation

Pioneering studies on the ability of extracts from decalcified bone matrix to promote ectopic bone and cartilage formation [1] led to searches for the identity of these morphogens which define

Expression of bone morphogenetic proteins and rat distal-less homolog genes following rat femoral fracture

Findings indicate that BMP-4 and rDlx are selectively expressed following femoral fracture in the rat, and suggest that they are involved in the formation of the callus at an early point during the postfracture healing of bone.

Bone Morphogenetic Protein Receptor IB Signaling Mediates Apoptosis Independently of Differentiation in Osteoblastic Cells*

BMP-2-induced apoptosis was mediated by BMP-RIB in osteoblasts and occurred independently of B MP- 2-induced osteoblast differentiation, which provides additional insights into the dual mechanism of Bmp-2 action on osteoblow fate.



Recombinant human bone morphogenetic protein 2B stimulates PC12 cell differentiation: potentiation and binding to type IV collagen

Direct experiments demonstrated that radioiodinated BMP 2B bound to collagen type IV better than to either laminin or fibronectin, and suggest that these regulatory molecules alone and in conjunction with extracellular matrix components may play a role in both the development and repair of nervous tissue.

Identification of type I receptors for osteogenic protein-1 and bone morphogenetic protein-4.

Results suggest thatALK-3 and ALK-6 are type I receptors for OP-1 and BMP-4; in addition, ALk-2 is a type I receptor shared by activin and OP- 1, but not by B MP-4.

Cloning and characterization of a human type II receptor for bone morphogenetic proteins.

The cDNA cloning and characterization of a human type II receptor for BMPs (BMPR-II) is reported, which is distantly related to DAF-4, a BMP type II receptors from Caenorhabditis elegans.

Molecular cloning of rat bone morphogenetic protein (BMP) type IA receptor and its expression during ectopic bone formation induced by BMP.

A cDNA for the rat bone morphogenetic protein (BMP) type IA receptor (BMPR-IA) was isolated from a dental pulp cell cDNA library and reverse transcriptase-polymerase chain reaction analysis revealed that BMPR- IA mRNA was highly expressed in the BMP-induced bone forming tissues throughout the stages tested.

Distinct spatial and temporal expression patterns of two type I receptors for bone morphogenetic proteins during mouse embryogenesis.

The complementary DNA cloning of the mouse homolog of ALK-3 is reported, which is highly conserved between mouse and man, and the expression in sites of developing cartilage and bone supports the idea that ALK3 and -6 are receptors for BMPs in vivo.

Localization of osteogenic protein-1 (bone morphogenetic protein-7) during human embryonic development: high affinity binding to basement membranes.

Findings suggest that, in addition to bone formation, OP-1 could have other important regulatory roles in human embryogenesis with high binding affinity to a basement membrane component.

Transient and localized expression of bone morphogenetic protein 4 messenger RNA during fracture healing

  • T. NakaseS. Nomura K. Takaoka
  • Biology
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
  • 1994
The results suggest that newly produced BMP‐4 gene product is one of the local contributing factors in callus formation in the early phase of fracture healing.

Novel regulators of bone formation: molecular clones and activities.

Human complementary DNA clones corresponding to three polypeptides present in this BMP preparation have been isolated, and expression of the recombinant human proteins have been obtained, and each appears to be independently capable of inducing the formation of cartilage in vivo.

Recombinant human bone morphogenetic protein induces bone formation.

  • E. WangV. Rosen P. LaPan
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1990
The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts, and has therapeutic potential to promote de novo bone formation in humans.

Osteogenin and recombinant bone morphogenetic protein 2B are chemotactic for human monocytes and stimulate transforming growth factor beta 1 mRNA expression.

It is demonstrated that at concentrations of 10-100 fg/ml (0.3-3 fM), native bovine osteogenin and recombinant human BMP-2B (rhBMP- 2B) induce the directed migration of human blood monocytes in vitro, associated with expression of BMP binding sites (receptors) on monocytes.