Enhanced expression of codon optimized interferon gamma in CHO cells.

@article{Chung2013EnhancedEO,
  title={Enhanced expression of codon optimized interferon gamma in CHO cells.},
  author={Bevan Kai-Sheng Chung and Faraaz Noor Khan Yusufi and Mariati and Yuansheng Yang and Dong-Yup Lee},
  journal={Journal of biotechnology},
  year={2013},
  volume={167 3},
  pages={326-33}
}
The human interferon-gamma (IFN-γ) is a potential drug candidate for treating various diseases due to its immunomodulatory properties. The efficient production of this protein can be achieved through a popular industrial host, Chinese hamster ovary (CHO) cells. However, recombinant expression of foreign proteins is typically suboptimal possibly due to the usage of non-native codon patterns within the coding sequence. Therefore, we demonstrated the application of a recently developed codon… CONTINUE READING