Enhanced expression of a peanut agglutinin reactive O linked oligosaccharide on fibronectins from the synovial fluid of patients with rheumatic disease: quantitation, domain localization, and functional significance.

OBJECTIVE To characterize the domain localization, quantitation, and functional binding consequences of an O linked oligosaccharide expressed on synovial fluid (SF) fibronectin (Fn). METHODS Identification and localization of the O linked oligosaccharide was performed by limited digestion of isolated SF Fn with a series of proteolytic enzymes followed by… CONTINUE READING