Enhanced expression, native purification, and characterization of CCR5, a principal HIV-1 coreceptor.

@article{Mirzabekov1999EnhancedEN,
  title={Enhanced expression, native purification, and characterization of CCR5, a principal HIV-1 coreceptor.},
  author={Tajib A. Mirzabekov and Norbert Bannert and Michael Farzan and Werner Hofmann and Peter Kolchinsky and Lin Wu and Richard Wyatt and Joseph Sodroski},
  journal={The Journal of biological chemistry},
  year={1999},
  volume={274 40},
  pages={28745-50}
}
Seven-transmembrane segment, G protein-coupled receptors (GPCRs) play important roles in many biological processes in which pharmaceutical intervention may be useful. High level expression and native purification of GPCRs are important steps in the biochemical and structural characterization of these molecules. Here, we describe enhanced mammalian cell expression and purification of a codon-optimized variant of the chemokine receptor CCR5, a GPCR that plays a central role in the entry of the… CONTINUE READING

From This Paper

Topics from this paper.
39 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 39 extracted citations

Similar Papers

Loading similar papers…