Enhanced ex vivo expansion of human adipose tissue-derived mesenchymal stromal cells by fibroblast growth factor-2 and dexamethasone.

@article{Lee2009EnhancedEV,
  title={Enhanced ex vivo expansion of human adipose tissue-derived mesenchymal stromal cells by fibroblast growth factor-2 and dexamethasone.},
  author={Sun-Young Lee and Jiwon Lim and Gilson Khang and Youngsook Son and P. Choung and S Kang and So Young Chun and H I Shin and Shin-Yoon Kim and Eui Kyun Park},
  journal={Tissue engineering. Part A},
  year={2009},
  volume={15 9},
  pages={2491-9}
}
In the present study, we investigated the ex vivo expansion of human adipose tissue-derived mesenchymal stromal cells (ATSCs) to identify factors that promoted efficient expansion while preserving stem cell potential. We examined several growth factors and steroids, and found that the combination of a low concentration of fibroblast growth factor-2 (FGF-2) (1 ng/mL) and dexamethasone (DEX) or betamethasone (BET) enhanced the proliferation of ATSCs by approximately 30-60% as compared to control… CONTINUE READING