Enhanced differentiation of embryonic stem cells using co-cultivation with hepatocytes.

@article{Moore2008EnhancedDO,
  title={Enhanced differentiation of embryonic stem cells using co-cultivation with hepatocytes.},
  author={Rebecca N Moore and Anouska Dasgupta and Nayyereh Rajaei and Martin L. Yarmush and Mehmet Toner and Lionel Larue and Prabhas V. Moghe},
  journal={Biotechnology and bioengineering},
  year={2008},
  volume={101 6},
  pages={1332-43}
}
We examined the effects of co-cultivated hepatocytes on the hepatospecific differentiation of murine embryonic stem (ES) cells. Utilizing an established mouse ES cell line expressing high or low levels of E-cadherin, that we have previously shown to be responsive to hepatotrophic growth factor stimulation (Dasgupta et al., 2005. Biotechnol Bioeng 92(3):257-266), we compared co-cultures of cadherin-expressing ES (CE-ES) cells with cultured rat hepatocytes, allowing for either paracrine… CONTINUE READING