Enhanced detection of surface-associated bacteria in indoor environments by quantitative PCR.

  title={Enhanced detection of surface-associated bacteria in indoor environments by quantitative PCR.},
  author={Mark P. Buttner and P Cruz-Perez and Linda D. Stetzenbach},
  journal={Applied and environmental microbiology},
  volume={67 6},
Methods for detecting microorganisms on surfaces are needed to locate biocontamination sources and to relate surface and airborne concentrations. Research was conducted in an experimental room to evaluate surface sampling methods and quantitative PCR (QPCR) for enhanced detection of a target biocontaminant present on flooring materials. QPCR and culture analyses were used to quantitate Bacillus subtilis (Bacillus globigii) endospores on vinyl tile, commercial carpet, and new and soiled… CONTINUE READING
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