Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.

@article{Kawarasaki2003EnhancedCS,
  title={Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.},
  author={Yasuaki Kawarasaki and Karl E. Griswold and James D Stevenson and Tzvia Selzer and Stephen J Benkovic and Brent L. Iverson and George D. Georgiou},
  journal={Nucleic acids research},
  year={2003},
  volume={31 21},
  pages={e126}
}
SCRATCHY is a methodology for the construction of libraries of chimeras between genes that display low sequence homology. We have developed a strategy for library creation termed enhanced crossover SCRATCHY, that significantly increases the number of clones containing multiple crossovers. Complementary chimeric gene libraries generated by incremental truncation (ITCHY) of two distinct parental sequences are created, and are then divided into arbitrarily defined sections. The respective sections… CONTINUE READING
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