Enhanced condensation and facilitated release of DNA using mixed cationic agents: a combined experimental and Monte Carlo study.

@article{Jorge2012EnhancedCA,
  title={Enhanced condensation and facilitated release of DNA using mixed cationic agents: a combined experimental and Monte Carlo study.},
  author={Andreia F Jorge and Rita S. Dias and Alberto A C C Pais},
  journal={Biomacromolecules},
  year={2012},
  volume={13 10},
  pages={3151-61}
}
Efficient DNA condensation and decondensation, as well as low toxicity, are required for an efficient gene delivery vehicle. We report on the condensation of DNA by a mixture of cationic agents, low-molecular-weight polyethylenimine (PEI, 1.2 KDa) and Fe(III) ions, and respective decondensation, using experimental and theoretical methods. It was found that a significant reduction in the amount of PEI necessary to induce DNA condensation is achieved by the addition of the trivalent ions, which… CONTINUE READING