Enhanced chemiluminescent reactions catalyzed by horseradish peroxidase.

  title={Enhanced chemiluminescent reactions catalyzed by horseradish peroxidase.},
  author={Gary Thorpe and Larry J. Kricka},
  journal={Methods in enzymology},
Enhancement of light emission from the horseradish peroxidase-catalyzed oxidation of diacyl hydrazides on addition of 6-hydroxybenzothiazole or phenol derivatives forms the basis of rapid, specific, and sensitive chemiluminescent assays for peroxidase. The advantages and wide applicability of the technique have been demonstrated in a range of ligand-binding assays. Careful selection of chemiluminescent reagents, enhancer, their relative proportions and reaction conditions, and more detailed… CONTINUE READING

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