Enhanced chemiluminescence labeled-antibody immunoassay (Amerlite-MAB) for free thyroxine: design, development, and technical validation.

@article{Christofides1995EnhancedCL,
  title={Enhanced chemiluminescence labeled-antibody immunoassay (Amerlite-MAB) for free thyroxine: design, development, and technical validation.},
  author={Nicholas D Christofides and C P Sheehan},
  journal={Clinical chemistry},
  year={1995},
  volume={41 1},
  pages={17-23}
}
We describe the development and validation of a one-step nonradioactive immunoassay for free thyroxine (Amerlite-MAB FT4) in serum or plasma, in the dedicated Amerlite enhanced luminescence assay system. A monoclonal antibody to thyroxine (T4), conjugated with horseradish peroxidase (HRP; EC 1.11.1.7), competes for binding with FT4 or with a conjugate of a protein and L-triiodothyronine immobilized onto the surface of the assay microwells. The signal is generated by antibody-HRP bound to the… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 41 references

In vitro and in vivo effects of increased concentrations of free fatty acids on free thyroxin measurements as determined by five assays

R Sapin, JL Schilenger, F Grunenberger, F Gasser, J. Chambron
Clin Chem • 1990
View 4 Excerpts
Highly Influenced

Evidence that NT! induces temporary hyperthyroidism [Abstract

N Boye, J. Weeke
Ann Endocrinol (Paris) 1986;47:29 • 1995
View 1 Excerpt

Liewendahl BK New ultrafiltration method for free thyroxun compared with equilibrium dialysis in patients with thyroid dysfunction and nonthyroidal illness

SH Tikanoja
Clin Chem • 1990
View 1 Excerpt

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