Enhanced Patient Serum Immunoreactivity to Recombinant Mycobacterium tuberculosis CFP32 Produced in the Yeast Pichia pastoris Compared to Escherichia coli and Its Potential for Serodiagnosis of Tuberculosis

@article{Benabdesselem2006EnhancedPS,
  title={Enhanced Patient Serum Immunoreactivity to Recombinant Mycobacterium tuberculosis CFP32 Produced in the Yeast Pichia pastoris Compared to Escherichia coli and Its Potential for Serodiagnosis of Tuberculosis},
  author={Chaouki Benabdesselem and Dahmani M. Fathallah and Richard C. Huard and Hongxia Zhu and Mohamed Ali Jarboui and Koussay Dellagi and John L. Ho and Ridha M Barbouche},
  journal={Journal of Clinical Microbiology},
  year={2006},
  volume={44},
  pages={3086 - 3093}
}
ABSTRACT CFP32 is a Mycobacterium tuberculosis complex-restricted secreted protein that was previously reported to be present in a majority of sputum samples from patients with active tuberculosis (TB) and to stimulate serum antibody production. CFP32 (originally annotated as Rv0577 and also known as TB27.3) was therefore considered a good candidate target antigen for the rapid serodiagnosis of TB. However, the maximal sensitivity of CFP32 serorecognition may have been limited in earlier… 

Proteomic profile of culture filtrate from the Brazilian vaccine strain Mycobacterium bovis BCG Moreau compared to M. bovis BCG Pasteur

Two-dimensional electrophoresis and mass spectrometry are combined to analyse the proteomic profile of culture filtrate proteins from M. bovis BCG Moreau, revealing aspects that may lead to a better understanding of the factors leading to BCG's variable protective efficacy against TB.

Immunogenicity of heparin-binding hemagglutinin expressed by Pichia pastoris GS115 strain

Hemagglutinin with no label has excellent immunogenicity, and is suitable for evaluating the effectiveness to prevent M. tuberculosis infection.

Serological Evaluation of Mycobacterium ulcerans Antigens Identified by Comparative Genomics

Six of the seven proteins identified showed an ability to distinguish people living in an endemic area from those in a non-endemic area with an average sensitivity of 69% and specificity of 88%, suggesting exposure to M. ulcerans.

Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection

The rPstS-1 could be used as a tool for studying the role of this molecule during Mtb infection, and to develop and improve vaccines or kits based on the recombinant protein for serodiagnosis.

Signal peptide prediction suggests Mycobacterium tuberculosis curli pilin subunit secretion via the Sec pathway may hinder MTP overexpression in Escherichia coli

Excluding the signal peptide region and using a GST tag greatly enhanced the expression of the protein in the soluble fraction and purification of the M. tuberculosis peptide remained problematic due to a lower available peptide concentration resulting from the lower molecular weight, in the purified fraction compared to the GST tag.

The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosis

Aptamer of MPT64 as a new detection tool, to a certain extent, is feasible to diagnose Mycobacterium tuberculosis.

Improved immunogenicity of mycobacterium tuberculosis Rv 0577 by a heterologous prime-boost vaccination strategy in mice

The results showed that the improved immunogenicity can be elicited by Rv0577 with an appropriate vaccination strategy, and it could be considered for developing a novel vaccine against TB.

Granzyme B induced by Rv0140 antigen discriminates latently infected from active tuberculosis individuals

Granzyme B is proposed as a host marker to help identify LTBI individuals and, in response to Rv0140, granzyme B seems to allow better discrimination of LTBI from aTB with areas under the curve (AUC) of 0.88.

The Mycobacterium bovis BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice.

The findings suggest that antigen Rv0577-based DNA vaccine is immunogenic and can efficiently boost BCG, which could be helpful in the design of an efficient vaccination strategy against TB.

References

SHOWING 1-10 OF 41 REFERENCES

High level expression of recombinant Mycobacterium tuberculosis culture filtrate protein CFP32 in Pichia pastoris

These data are the first to show that P. pastoris is a suitable host for high-yield production of good quality mycobacterium antigens, and especially culture filtrate proteins that have vaccine and diagnostic potential.

Expression of Mycobacterium tuberculosis MPT64 in recombinant Myco. smegmatis: purification, immunogenicity and application to skin tests for tuberculosis

A unique recombinant mycobacterial vector which secretes the encoded Myco.

Delineation of human antibody responses to culture filtrate antigens of Mycobacterium tuberculosis.

Studies suggest that 3 of the antigens recognized by sera from patients with early TB (85C, MPT32, and a 88-kDa protein) have strong serodiagnostic potential.

Decreased Capacity of Recombinant 45/47-kDa Molecules (Apa) ofMycobacterium tuberculosis to Stimulate T Lymphocyte Responses Related to Changes in Their Mannosylation Pattern*

The recombinant Apa expressed in Mycobacterium smegmatis was 4-fold less potent in vivo in the DTH assay and 10-fold more active in vitro to stimulate sensitized T lymphocytes than the native proteins, and the observed biological effects were related to the extent of glycosylation of the antigen.

Combinatorial Use of Antibodies to Secreted Mycobacterial Proteins in a Host Immune System-Independent Test for Tuberculosis

The results indicate that elevated Ag85 levels can be detected in patients with active tuberculosis even after BCG vaccination and suggest that combinatorial use of antibodies directed at different epitopes of this protein could provide a viable strategy for developing new host immune response-independent diagnostic tests for tuberculosis.

Homogeneity of Antibody Responses in Tuberculosis Patients

The mapping with individual patient sera presented here suggests that serodiagnostic tests based on the subset of antigens recognized during both noncavitary and cavitary TB will enhance the sensitivity of antibody detection in TB patients, especially in difficult-to-diagnose, smear-negative, nonc Cavitary TB patients.

Assessing the Serodiagnostic Potential of 35 Mycobacterium tuberculosis Proteins and Identification of Four Novel Serological Antigens

The aim of the present study was to identify novel serological targets for use for the future serodiagnosis of tuberculosis (TB), and identified four superior seroantigens, none of which has been described before.

Cloning and Expression of Immunoreactive Antigens from Mycobacterium tuberculosis

All of these recombinant antigens demonstrated potential for serodiagnosis of tuberculosis, and the B.6 antigen showed preferential reactivity to antibodies in pulmonary compared to nonpulmonary tuberculosis serum specimens.

Serodiagnostic Potential of Culture Filtrate Antigens of Mycobacterium tuberculosis

The results demonstrate that antigens identified on the basis of their reactivity with TB patients' sera provide high sensitivities for serodiagnosis and reaffirm that both the extent of the disease and the bacterial load may play a role in determining the antigen profile recognized by antibodies.