Enhanced PCR amplification of VNTR locus D1S80 using peptide nucleic acid (PNA).

@article{Demers1995EnhancedPA,
  title={Enhanced PCR amplification of VNTR locus D1S80 using peptide nucleic acid (PNA).},
  author={D B Demers and Edward Curry and Michael Egholm and Amanda Sozer},
  journal={Nucleic acids research},
  year={1995},
  volume={23 15},
  pages={3050-5}
}
Use of the polymerase chain reaction (PCR) to amplify variable numbers of tandem repeat (VNTR) loci has become widely used in genetic typing. Unfortunately, preferential amplification of small allelic products relative to large allelic products may result in incorrect or ambiguous typing in a heterozygous sample. The mechanism for preferential amplification has not been elucidated. Recently, PNA oligomers (peptide nucleic acids) have been used to detect single base mutations through PCR… CONTINUE READING
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