Enhanced FK506 production in Streptomyces clavuligerus CKD1119 by engineering the supply of methylmalonyl-CoA precursor

  title={Enhanced FK506 production in Streptomyces clavuligerus CKD1119 by engineering the supply of methylmalonyl-CoA precursor},
  author={SangJoon Mo and Yeon-Hee Ban and J. Park and Y. J. Yoo and Y. Yoon},
  journal={Journal of Industrial Microbiology & Biotechnology},
  • SangJoon Mo, Yeon-Hee Ban, +2 authors Y. Yoon
  • Published 2009
  • Biology, Medicine
  • Journal of Industrial Microbiology & Biotechnology
  • FK506 is a 23-membered polyketide macrolide with immunosuppressant activity produced by Streptomyces species. The production of FK506 in S. clavuligerus CKD1119 (KCTC 10561BP) was improved by enhancing the supply of biosynthetic precursors. This improvement was approximately 2.5-fold (15 mg/l) with the supplementation of 10 mM methyl oleate, which is the probable source of acyl-CoAs, to R2YE medium. When the level of FK506 production reached its maximum, the intracellular concentration of… CONTINUE READING
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