Enhanced Detection of Tuberculous Mycobacteria in Animal Tissues Using a Semi-Nested Probe-Based Real-Time PCR

@inproceedings{Costa2013EnhancedDO,
  title={Enhanced Detection of Tuberculous Mycobacteria in Animal Tissues Using a Semi-Nested Probe-Based Real-Time PCR},
  author={Pedro Alexandre Costa and Ana S D Ferreira and Ana Amaro and Teresa Albuquerque and Ana Botelho and Isabel Couto and M{\^o}nica Cunha and Miguel Viveiros and Jo{\~a}o In{\'a}cio},
  booktitle={PloS one},
  year={2013}
}
Bovine tuberculosis has been tackled for decades by costly eradication programs in most developed countries, involving the laboratory testing of tissue samples from allegedly infected animals for detection of Mycobacterium tuberculosis complex (MTC) members, namely Mycobacterium bovis. Definitive diagnosis is usually achieved by bacteriological culture, which may take up to 6-12 weeks, during which the suspect animal carcass and herd are under sanitary arrest. In this work, a user-friendly DNA… CONTINUE READING