Enhanced BRET Technology for the Monitoring of Agonist-Induced and Agonist-Independent Interactions between GPCRs and β-Arrestins

@inproceedings{Kocan2010EnhancedBT,
  title={Enhanced BRET Technology for the Monitoring of Agonist-Induced and Agonist-Independent Interactions between GPCRs and β-Arrestins},
  author={Martina Kocan and Matthew B. Dalrymple and Ruth M. Seeber and Brian J. Feldman and Kevin Donald George Pfleger},
  booktitle={Front. Endocrin.},
  year={2010}
}
The bioluminescence resonance energy transfer (BRET) technique has become extremely valuable for the real-time monitoring of protein-protein interactions in live cells. This method is highly amenable to the detection of G protein-coupled receptor (GPCR) interactions with proteins critical for regulating their function, such as β-arrestins. Of particular interest to endocrinologists is the ability to monitor interactions involving endocrine receptors, such as orexin receptor 2 or vasopressin… CONTINUE READING